Autologous periodontal ligament stem cells combined with composites repair periodontal bone defects in miniature pigs

doi:10.3969/j.issn.2095-4344.2013.16.001

Abstract

Abstract:

BACKGROUND: Studies have proved that autologous periodontal ligament stem cells combined with scaffold materials can achieve better effect on the repair of periodontal bone defects.
OBJECTIVE: To observe the effect of miniature pig autologous periodontal ligament stem cells combined with hydroxyapatite bioceramic composites in the repair of category Ⅱ periodontal bone defects.
METHODS: Six Guizhou miniature pigs were included, and were used to establish the miniature pig models of upper and lower jaw category Ⅱ periodontal bone defects. The bone defects were located between the third premolar and fourth premolar, and the near root of fourth premolar was exposed. The defects in the experimental group were repaired with periodontal ligament stem cells obtained from the autologous maxillary and mandibular canine and combined with hydroxyapatite bioceramic; the defects in the control group were repaired with hydroxyapatite bioceramic simplely; and the model group did not repaired. The defects in each group were covered with oral biofilm. At 12 weeks after modeling, the defect periodontal tissues were obtained from each group, and then the osteogenesis healing of the periodontal bone tissue was observed through clinical observation, heal spiral CT scanning, three-dimensional reconstruction and hematoxylin-eosin staining.
RESULTS AND CONCLUSION: Clinical observation showed that healing of periodontal defects in the experimental group was the best. Head spiral CT scanning showed that there was no significant difference of bone mineral density between bone defect area and surrounding normal alveolar bone, and the defects in the control group and the model group were still clear visible in the incomplete healing state. Hematoxylineosin staining showed that the defect area in the experimental group was filled with newborn alveolar bone completely, and the normal calcified bone structure was established; in the control group, the defect area was filled with newborn alveolar bone incompletely with low calcification degree; a large number of collagen fibers could be seen in the model group, and the defect area was not filled and no new bone formed. It is visible that periodontal ligament stem cells combined with guided tissue regeneration can effectively promote healing of category II periodontal bone defects and periodontal regeneration in miniature pigs, thus repairing periodontal bone defects.

Key words: biomaterials, tissue-engineered oral materials, stem cell transplantation, periodontal ligament stem cells, guided tissue regeneration, osteogenesis, periodontal regeneration, bone defect, periodontal disease, provincial grants-supported paper

CLC Number:

R318

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R318

Yang Si-hui, Zhong Liang-jun, Zhang Peng-tao,Zhang Yuan,Zhang Yuan-ming,Ma Lu-ping. Autologous periodontal ligament stem cells combined with composites repair periodontal bone defects in miniature pigs[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(16): 2851-2858.

Figures/Tables 4

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